Publications

  1. Vaidyanathan S, Kerschner JL, Paranjapye A, Sinha V, Lin B, Bedrosian TA, Thrasher AJ, Turchiano G, Harris A, Porteus MH. Investigating adverse genomic and regulatory changes caused by replacement of the full-length CFTR cDNA using Cas9 and AAV. Molecular Therapy-Nucleic Acids. 2024 Mar 12;35(1). DOI:https://doi.org/10.1016/j.omtn.2024.102134

  2. Selvaraj S, Feist WN, Viel S, Vaidyanathan S, Dudek AM, Gastou M, Rockwood SJ, Ekman FK, Oseghale AR, Xu L, Pavel-Dinu M, Luna SE, Cromer MK, Sayana R, Gomez-Ospina N, Porteus MH. High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition. Nature Biotechnology. 2023 pp.1-14.

  3. Vaidyanathan S, Trumbull AM, Bar L, Rao M, Yu Y, Sellers ZM. CFTR genotype analysis of Asians in international registries highlights disparities in the diagnosis and treatment of Asian patients with cystic fibrosis. Genetics in Medicine. 2022. https://doi.org/10.1016/j.gim.2022.06.009.

  4. Vaidyanathan S, Baik R, Chen L, Bravo D.T, Suarez C. J, Abazari S.M,  Salahudeen A.A, Dudek A.M, Teran C.A, Davis T.H, Lee C.M, Bao G, Randell S.H, Artandi S.E, Wine J.J, Kuo C.J, Desai T.J, Nayak J.V, Sellers Z.M, Porteus M.H. Targeted Replacement of Full-Length CFTR in Human Airway Stem Cells By CRISPR/Cas9 For Pan-Mutation Correction in the Endogenous Locus. Molecular Therapy. 2021. DOI: 10.1016/j.ymthe.2021.03.023

  5. Bonafont J, Mencía A, Chacón-Solano E, Srifa W, Vaidyanathan S, Romano R, Garcia M, Hervás-Salcedo R, Ugalde L, Duarte B, Porteus M.H, Del Rio M, Larcher F, Murillas R. Correction of Recessive Dystrophic Epidermolysis Bullosa by Homology-directed Repair-mediated Genome Editing. Molecular Therapy. 2021. DOI: 10.1016/j.ymthe.2021.02.019

  6. Vaidyanathan S, Salahudeen A.A, Sellers Z.S, Bravo D.T, Choi S.S, Batish A, Le W, De La O S, Kaushik M.P, Galper N, Lee C.M, Bao G, Chang E.H, Wine J.J, Milla C.E,  Desai T.J, Nayak J.V, Kuo C.J, Porteus M.H. High-Efficiency, Selection-Free Gene Repair in Airway Stem Cells from Cystic Fibrosis Patients Rescues CFTR Function in Differentiated Epithelia. Cell Stem Cell. 2020. DOI: 10.1016/j.stem.2019.11.002

  7. Martin R.M, Ikeda K, Cromer M.K, Uchida N, Nishimura T, Romano R, Tong A.J, Lemgart V.T, Camarena J, Pavel-Dinu M, Sindhu C, Wiebking V, Vaidyanathan S, Dever D.P, Bak R.O, Laustsen, Lesch B.J, Jakobsen M.R, Sebastino V, Nakauchi H, Porteus M.H. Highly efficient and marker-free genome editing of human pluripotent stem cells by CRISPR-Cas9 RNP and AAV6 donor-mediated homologous recombination. Cell Stem Cell. 2019. DOI: 10.1016/j.stem.2019.04.001

  8. Vaidyanathan S, Azizian K.T, Haque A.A*, Henderson J.M, Hendel A, Shore S, Antony J.S, Hogrefe R.I, Kormann M.S, Porteus M.H and McCaffrey A.P. Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity Without HPLC Purification. Molecular Therapy-Nucleic Acids. 2018.
    DOI: 10.1016/j.omtn.2018.06.010

  9. Cromer M.K, Vaidyanathan S, Ryan D.E, Curry B, Lucas A.B, Camarena J.C, Kaushik M.P, Hay S.R, Martin R.M, Steinfeld I, Bak R.O, Dever D.P, Hendel A, Bruhn L and Porteus M.H. Global Transcriptional Response to CRISPR/Cas9-AAV6-Based Genome Editing in CD34+ Hematopoietic Stem and Progenitor Cells. Molecular Therapy, 2018.
    DOI: 10.1016/j.ymthe.2018.06.002

  10. Camarena J.C, Charlesworth C.T, Cromer M.K, Vaidyanathan S, Bak R.O, Carte J, Potter J, Dever D.P and Porteus M.H. Priming Human Hematopoietic Stem and Progenitor Cells for Cas9/SgRNA and rAAV6-Mediated Homologous Recombination. Molecular Therapy-Nucleic Acids. 2018. DOI: 10.1016/j.omtn.2018.04.017

  11. Vaidyanathan S, Kaushik M.P, Dougherty C.A, Rattan R, Goonawardena S.N, Banaszak Holl M.M, Monano J, DiMaggio S. Increase in Dye: Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells. ACS Biomaterials Science and Engineering. 2016. DOI: 10.1021/acsbiomaterials.6b00308

  12. Vaidyanathan S, Chen J , Orr B.G and Banaszak Holl M.M. Cationic Vector Intercalation into the Lipid Membrane Enables Intact Polyplex DNA Escape from Endosomes for Gene Delivery.  Molecular Pharmaceutics. 2016. DOI: 10.1021/acs.molpharmaceut.6b00139

  13. Vaidyanathan S, Merzel R.L, Banaszak Holl M.M. et al. Quantitative Measurement of Cationic Polymer Vector and Polymer/pDNA Polyplex Intercalation into the Cell Plasma Membrane. ACS Nano. 2015. DOI: 10.1021/acsnano.5b01263

  14. Dougherty, C.A, Vaidyanathan S and Banaszak Holl M.M. Fluorophore: Dendrimer Ratio Impacts Cellular Uptake and Fluorescence Lifetime. Bioconjugate Chemistry. 2015.
    DOI: 10.1021/bc5005735

  15. Vaidyanathan S, Orr B. G , & Banaszak Holl M. M. Detergent Induction of HEK 293A Cell Membrane Permeability Measured Under Quiescent and Superfusion Conditions using Whole Cell Patch Clamp. Journal of Physical Chemistry B. 2014. DOI: 10.1021/jp4124315

  16. van Dongen M.A, Vaidyanathan S and Banaszak Holl M.M. PAMAM dendrimers as quantized building blocks for novel nanostructures. Soft Matter. Oct 2013. DOI: 10.1039/C3SM52250D

  17. Rattan R, Vaidyanathan S, Wu G. S.-H., et al. Polyplex-Induced Cytosolic Nuclease Activation Leads to Differential Transgene Expression. Molecular Pharmaceutics. 2013.
    DOI: 10.1021/mp400103f

  18. Matz R. L., Erickson B, Vaidyanathan S, et al. Polyplex Exposure Inhibits Cell Cycle, Increases Inflammatory Response, and Can Cause Protein Expression without Cell Division. Molecular Pharmaceutics. 2013. DOI: 10.1021/mp300470d

  19. Richards D.N, Zemlyanov D.Y, Vaidyanathan S, Ivanisevic A et al. DNA Immobilization on GaP (100) Investigated by Kelvin Force Probe Microscopy. 
    Journal of Physical Chemistry C. 2010. DOI: https://doi.org/10.1021/jp105927t

  20. Vaidyanathan S, McCarra M and Desai T.J. Lung stem cells and therapy for cystic fibrosis. Lung Stem Cells in Development, Health and Disease (ERS Monograph). 2021.

  21. Vaidyanathan S, Orr B.G and Banaszak Holl M.M. Role of Cell Membrane–Vector Interactions in Successful Gene Delivery. Accounts of Chemical Research.2016.
    DOI: 10.1021/acs.accounts.6b00200